HMB-45 AND KI-67 STAINING FOR MELANOMA ASSOCIATED WITH NEVI

  • Joana Gomes Serviço de Dermatologia, Hospital de Braga
  • Joana Parente Serviço de Dermatologia, Hospital de Santarém EPE
  • Isabel Viana Centro de Dermatologia Médico-Cirúrgica de Lisboa
  • Esmeralda Vale Centro de Dermatologia Médico-Cirúrgica de Lisboa
Keywords: Melanoma, Nevi, HMB-45, Ki-67, Immunohistochemestry

Abstract

Introduction: The majority of melanomas appear to arise de novo, however in about one third of the cases it can arise in association with a preexisting nevus. The histopathological diagnosis in these situations can be difficult, particularly in the separation of the two cellular populations and, as a consequence, establishing the tumoral thickness. Immunohistochemical staining can help in this distinction.

Objectives: To evaluate the immunohistochemical staining patterns of HMB-45 and Ki-67 in selected cases of malignant melanoma associated with melanocytic nevi.

Methods: Immunoperoxidase stains for HMB-45 and Ki-67 were done on 5 dermal melanocytic nevi, 5 compound melanocytic nevi, 5 invasive melanomas and 16 melanomas arising in a nevus.

Results: The dermal melanocytes of nevi were consistently negative for HMB-45 and Ki-67. The melanomas showed diffuse moderate to intense staining for HMB-45 and Ki-65. In melanomas associated with nevus the HMB-45 was consistently positive in the melanoma component and negative in the nevus, allowing the separation of the two cellular populations. We find a considerable variation in the Ki-67 staining in this group.

Conclusion: Although the histopathological examination with hematoxilin and eosin remains the main component in the diagnosis of melanocytic lesions; the immunohistochemical staining with HMB-45 and Ki-67 can be helpful in the diagnosis of melanoma associated with nevi.

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Published
2011-06-23
How to Cite
Gomes, J., Parente, J., Viana, I., & Vale, E. (2011). HMB-45 AND KI-67 STAINING FOR MELANOMA ASSOCIATED WITH NEVI. Journal of the Portuguese Society of Dermatology and Venereology, 69(2), 195-201. https://doi.org/10.29021/spdv.69.2.605
Section
Original Articles